Flow Cytometry

Checking of antigens on the surface, inside the cytoplasm or cell nucleus (immunophenotyping)

Checking of apoptosis and cell necrosis

Checking of cell cycle phases (G0, G1, S, G2, M)


Applications of the device:

  • Checking of cell proliferation
  • Checking of granulite and cell size
  • Checking karyotype (flow karyotyping)


Abstract of the applications of the device

The analyzed cell sample is uniformly dispersed and enters a narrow column filled with sheet or sheath fluid by pressurized isotonic fluid. The sheet solution surrounds the narrow canal and the sample undergoes a central flow through the center of the sheet solution into individual cells one after the other. The test particles are suspended in a liquid and pass through a narrow aperture and in front of a narrow beam of laser light at a speed of about 5 to 50 meters per second, thus we can collect information about 5000 to 50000 cells per second. To identify antigens, the sample is adjacent to specific monoclonal antibodies labeled with one of the fluorochromes. If there is a reaction, the cell surface becomes fluorescent and is excited by absorbing laser light and reflects a longer wavelength. Recycled light is measured at different angles. The sensitivity of cell separation depends on the shape and intensity of the laser light. The measurement of the scattering or scattering of laser light along the axis of the irradiated or forward beam depends on the size of the cell. Fluorescence light recovery analysis after exposure to laser light at lateral angles and 90 degree angles provides characteristics of antigenic status and intracellular contents.


Cost of the test

Other universities and research centers (each sample from 1 to CD 3 markers)


(each sample from 1 to CD 3 markers)


Cellular level


Cellular level





The applicant is responsible for preparation of antibodies